Fig. 6From: Uncovering impaired mitochondrial and lysosomal function in adipose-derived stem cells from obese individuals with altered biological activityLysosomal morphology and function were impaired in O-ADSCs. a Immunofluorescence staining of L-ADSCs and O-ADSCs with antibodies against LC3 and LAMP1. Scale bar, 50 μm. b Representative lysosomal images of L-ADSCs and O-ADSCs stained with LysoTracker Red. Scale bar, 50 μm. c Representative TEM images of L-ADSCs and O-ADSCs. Red arrows indicate atypical lysosomes with some undegraded materials. Scale bar, 2 μm. b Immunofluorescence staining of L-ADSCs and O-ADSCs with antibodies against LC3 and LAMP1. Scale bar, 50 μm. c Representative lysosomal images of L-ADSCs and O-ADSCs stained with LysoTracker Red. Scale bar, 50 μm. d Phagocytosis of L-ADSC and O-ADSCs was measured by phagocytic FITC-Aβ after being treated with FITC-Aβ for 4 h. e Qualification of fluorescence(fluo-) intensity of FITC-Aβ per cell in L-ADSCs and O-ADSCs. At least 50 cells were assessed in each groupBack to article page