Fig. 1

Muscle regeneration in hindlimb ischemia after ADSCs delivery. A Representative images of transverse sections of the gastrocnemius muscles stained by H&E in: healthy, ischemic, intramuscular (i.m.), intravenous (i.v.) and contralateral (c.) groups on the 7th and the 14th days post-injury (dpi). B Number of muscle fibers counted in collected muscles on the 7th and the 14th dpi. The number of muscle fibers was determined in 5 fields of view per animal (n = 3–5) at a magnification of 20× and calculated per 1 mm² of tissue. C Ratio of regenerating myofibers (white arrows) counted in collected muscles on the 7th and the 14th dpi. Regenerating myofibers with two or more centralized nuclei was counted in 5 fields of view per animal (n = 3–5) at a magnification of 20×. Results are presented as mean ± standard error (SE) or in box plot as median, quartiles and min/max values as whiskers. #p < 0.02, ##p < 0.002 by the ANOVA followed by the Tukey’s post hoc test. **p < 0.02, ***p < 0.0001 evaluated with Kruskal–Wallis one-way analysis of variance and multiple comparison of mean ranks for all groups