Fig. 4

Influx of macrophages in hindlimb ischemic tissue after ADSCs delivery. A Representative images of M2 macrophages—double immunostaining of [F4/80⁺ (green), CD206⁺ (red)] in: healthy, ischemic, intramuscular, intravenous and contralateral groups on the 7th and the 14th days post-injury (dpi). Nuclei stained with DAPI (blue), scale bars = 50 μm. B Percentage of area occupied by macrophages (F4/80⁺ cells) was counted in collected muscles on the 7th and the 14th dpi. The total area occupied by F4/80⁺ cells was calculated using ImageJ software in 5 fields of view per animal (n = 3–5) at a magnification of 20×. Results are represented in box plot as median, quartiles and min/max values as whiskers. *p < 0.05, **p < 0.005, ***p < 0.0001 evaluated with Kruskal–Wallis one-way analysis of variance and multiple comparison of mean ranks for all groups