Fig. 1

Characterization of Osteo- and Naïve-EVs. a i and b i Histogram graphs depicting the average size of EVs particles (n = 3) in Osteo- and Naïve-EVs, measured by dynamic light scattering (DLS). a ii and b ii Representative transmission electron microscopy (TEM) images of Osteo-and Naïve-EVs (Magnification of the EVs in the main image x10k; scale bar = 2 µm, and in the small box x70K; scale bar = 200 nm). a iii and b iii Representative flow cytometry analysis of transmembrane proteins CD63, CD81, and CD9. b i Venn diagram across all 3 donors in the Osteo-EVs group and inter-donor comparison, and across the common Osteo-EV (882) proteins and both Vesiclepedia Top 100 proteins and Vesiclepedia datasets. c ii The enriched GO terms (BP, CC, and MF) for the common Osteo-EVs (882) proteins using FunRich tool (Version 3.1.3). d i Venn diagram across all 3 donors in the Naïve-EVs group and compared to one another, and across the common Naïve-EV (895) proteins and both Vesiclepedia Top 100 proteins and Vesiclepedia datasets. d ii The enriched GO terms (BP, CC, and MF) for the common Naïve-EVs (895) proteins using FunRich tool (Version 3.1.3)