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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Osteogenic human MSC-derived extracellular vesicles regulate MSC activity and osteogenic differentiation and promote bone regeneration in a rat calvarial defect model

Fig. 3

Effects of Osteo- and Naïve-EVs (10 µg/ml each) in cultured hMSC. a Representative images from the uptake experiments. Cultured hMSC internalised labelled Osteo- and Naïve-EVs: blue, DAPI-labelled nuclei; red, Phalloidin; green, DiOC18-labelled EVs. b Cell proliferation assay indicated that Osteo- and Naïve-EVs stimulate cultured hMSC proliferation after 24 and 72 h compared to the control medium group (BPS and medium). c i Representative images from the wound-healing experiments; scale bar 50 µm. Time course of cell migration wound-healing assay for cultured hMSC as the “recipient” cells with the control medium, Naïve- and Osteo-EVs. Scale bar 100 µm. c ii Quantification of the wound-healing assay from three independent donors. *p < 0.05, **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512