Osteogenic human MSC-derived extracellular vesicles regulate MSC activity and osteogenic differentiation and promote bone regeneration in a rat calvarial defect model

Table 1 Unique EV proteins and DEPs in both Osteo- and Naïve-EV groups

GO Name/Term	Matched protein in each EVs category		Osteo-EVs Naïve-EVs
GO Name/Term	Unique (19/137)	DEPs (8/27)	Unique (12/150)	DEPs (5/22)
Cell population proliferation GO:0008283 (176)	2 proteins: WNT5A, NOTCH2	2 proteins: CSF1, TGFB1	3 proteins: STAT3, CTNNB1, CAV1	4 proteins: ITGB1, GNAI2, RAP1B, GNB1
Cell migration GO:0016477 (133)	9 proteins: ARPC5, PDGFRA, WNT5A, ITGBL1, PAFAH1B1, IGFBP6, SDC1, SDC2, CDH2	4 proteins: AXL, SDC4, TGFB1, ATRN	5 proteins: ENG, JUP, CTNNA1, USP9Y, EPHA2	2 proteins: ITGB1, RRAS
BMP signaling pathway GO:0030509 (91)	3 proteins: TGFBR3, WNT5A, NOTCH2	2 proteins: MEGF8 TGFB1	2 proteins: ENG, USP9Y
Positive regulation of BMP signaling pathway GO:0030513 (43)	2 proteins: RNF165, NOTCH2	NUMA1	ENG
Bone mineralization GO:0030282 (54)	4 proteins: ENPP1, ANKH, MINPP1, ASPN		LOX
Notch Signaling pathway GO:0061314 (170)	3 proteins: NOTCH2, SUSD5, JAG1	2 proteins: TGFB1, APP	ANXA4
WNT Signaling pathway GO:0016055 (212)	2 proteins: WNT5A, WNT5B		3 proteins: CTNND1, CTNNB1, CPE

The table presents the unique EV proteins and DEPs in each group (i.e. corresponding gene names) included in the selected GO-terms obtained from QuickGO. The numbers given in parentheses (left column and in the right column in front of each QuickGO term) represent the total number of proteins specific to each GO term. Only protein symbols identified in Homo-sapiens species were retrieved and duplicate protein names were removed from the QuickGO list and EVs dataset. The numbers given in parentheses (beneath each EV category) represent the matched EV proteins compared to the total number of EV group

ISSN: 1757-6512