Fig. 3

Histological analysis of fat grafts in the GSH-treated and control (Con) groups. a Representative images of H&E-stained fat grafts at week 12. b H&E scoring of cell integrity, tissue inflammation, vacuolation, and fibrosis (n = 7). Asterisk, vacuoles. Triangle, fibrotic cells. Arrow, inflammatory cells c Immunofluorescence staining for perilipin (green) and von Willebrand factor (vWF, red) in fat grafts between treatment groups. The bottom images are the magnified fields contained in the above dotted frames d Quantification of positive perilipin and vWF staining based on mean fluorescence intensity (n = 3). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01