Fig. 4

Transcriptomic analysis of fat grafts from the GSH-treated and control (Con) groups. a Volcano plot of DEGs between the control and GSH groups (p < 0.05, fold change > 1.5 or < 0.667). b KEGG pathway enrichment analysis of DEGs. Ferroptosis was ranked third among the top-20 enriched pathways. c Gene set enrichment analysis of ferroptosis pathways. d Heatmap of genes involved in ferroptosis. Significant DEGs are shown in red. e Validation of DEGs by qRT-PCR. f Pearson’s correlation analysis of DEG fold-change levels determined by RNA-seq and qRT-PCR. Solid line indicates the fitting curve