Fig. 5

Effects of GSH supplementation on ferroptosis in fat grafts. a Immunofluorescence staining for GPX4 (green) and SLC7A11 (red) in fat grafts from control and GSH-treated mice. The bottom images are the magnified fields contained in the above dotted frames. b Mean fluorescence intensity of positive GPX4 and SLC7A11 staining (n = 3). c Ultrastructure of fat grafts based on TEM. The bottom images are the magnified fields contained in the above dotted frames (n = 3). Arrowheads indicate mitochondria. M: mitochondrion; LD: lipid drop. d, e Malondialdehyde (MDA) and superoxide dismutase (SOD) levels in control and GSH-treated grafts (n = 3). f Protein expression and quantification of GPX4, SLC7A11, and PPARγ in fat grafts (n = 3). Full-length blots are presented in Additional file 4. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01