Fig. 1

Scheme of hiPSC differentiation to oligodendrocytes. a Timeline of oligodendrocyte induction. b Morphology of the culture at different stages of differentiation from iPSCs to oligodendrocytes. Preparation of hiPSC cultures for differentiation (b1), cells were induced into OLIG2⁺ progenitors at day 12 (b2), then mechanically dissociated the cultures to form spheres during day 20 to day 30 (b3). After 45 days of differentiation, OPCs migrated out of the sphere and matured (b4). Scale bars, 100 μm. c Expression of PAX6 and OLIG2 at day 8 and day 12, as shown through immunofluorescence analysis (PAX6/OLIG2, red; GFP green; DAPI, blue). Scale bars, 100 μm. d O4⁺ and MBP⁺ stainings during the differentiation process. The proportion of O4⁺ and MBP⁺ OPCs gradually increased from 45 to 85 days (O4, red; MBP, white; DAPI, blue). Scale bars, 100 μm