Fig. 4

Survival, safety, and migration of engraftments in the SCI model 2 months post-transplantation. a Overview of GFP and Stem121 fluorescent immunolabeling demonstrates graft could survival, and migration 8 weeks post-grafting (Stem121, red; GFP, green). Scale bar, 500 μm. b OCT4 was negative in the transplanted cells (OCT4, white). Scale bar, 100 μm. c Grafts migrated to rostral and caudal of the host spinal cord, and the high-magnification view of the regions indicated by the inserts (Stem121, red; GFP, green). Scale bar, 2000 μm; high-magnification view, 100 μm. d The average migration distance of the co-transplantation group was 13.78 mm ± 0.48 mm, and the OPCs transplantation group was 9.47 mm ± 0.58 mm. The migration of the co-transplantation group was significantly enhanced (Stem121, red; GFP, green). Data are means ± SEM. *p < 0.05, **p < 0.01, t test, n = 5 rats in each group. The rats transplanted with OPCs alone were referred to as the OPCs-alone group, and those transplanted with OPCs combined with HUVECs were referred to as the co-transplantation group