Fig. 5

The tissue morphology, and grafts differentiation after 2 months of transplantation in vivo. a GFP⁺, NF200⁺ staining in the damaged area of each group after 8 weeks of transplantation. The high-magnification view of the regions was shown at the right side (GFP, green; NF200, red; GFAP, White). Scale bar, 500 μm (left panel), 75 μm (right panel). b Size of lesion areas in each group. The damaged area of transplantation groups was significantly decreased. Data are means ± SEM. *p < 0.05, **p < 0.01, One-way ANOVA, n = 6 rats in each group. Scale bar, 500 µm. c Compared with the NF200 pixel abundance in the lesion area of the control group, cell transplantation increased neurons density (NF200, red). Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, One-way ANOVA, n = 6 rats in each group. Scale bar, 75 µm. d Grafts differentiated into Tuj1/NF200/NF-L neurons, MBP oligodendrocytes, and GFAP astrocytes (GFP, green; Tuj1/NF200/NF-L/GFAP, red; MBP, white). n = 5. Scale bar, 100 μm