Fig. 6

Grafts contribute to remyelination and synapse formation 2 months post-transplantation in vivo. a MBP immunofluorescence staining showed demyelination/remyelineation in each group post-injury. The demyelination area was more extensive in the control group and fewer MBP⁺ cells in the lesion site than in the transplantation group, the high-magnification view of the regions indicated by the white box (GFP, green; MBP, White). Scale bar, 500 μm (left panel), 75 μm (right panel). b Statistics of demyelination areas in each group. Data are means ± SEM, ns, no significance; one-way ANOVA, n = 6 rats in each group. c Compared with the MBP pixel abundance of the control group in the lesion site, grafts promoted remyelination (MBP, white). Data are means ± SEM, ns, no significance; *p < 0.05, **p < 0.01, One-way ANOVA, n = 6 rats in each group. d GFP was co-labeled with Caspr/MBP (arrowheads), indicating the grafts formed nodes of Ranvier in vivo (GFP, green; Caspr, red; MBP, white). Scale bar, 25 μm. e The grafts form synapse (arrowheads) in vivo by co-staining of GFP and Synapsin (GFP, green; Synapsin, red). Scale bar, 50 μm