Fig. 5

Hematopoietic-specific NOD1 knockout ameliorates the progression of DR. A Study design for bone marrow transplantation. B Representative OCT images of retinal cross-sections (n = 10–12 per group). C Bar plots showing the quantification of retinal thickness of mice from diabetic and nondiabetic NOD1 chimeras as well as their controls. D Representative scotopic ERG waveforms from the chimera mice. E–H ERG amplitudes and implicit times of scotopic a-waves and b-waves (n = 14–16 per group). I Representative 12-Hz-flicker ERG waveforms. J The amplitudes of 12-Hz-flicker ERG in each group (n = 14 per group). K Retina vasculature was obtained through trypsin digestion and subsequently stained with PAS-hematoxylin. The presence of acellular capillaries, indicated by red arrows, is a characteristic pathological hallmark of DR. L Quantification of acellular capillaries in the respective groups (n = 15–17 per group). Data are presented as the mean ± SD; *p < 0.05, **p < 0.01. WT, wild type; OCT, optical coherence tomography; ERG, electroretinogram