Fig. 6

Reduced infiltration of bone marrow-derived macrophages in the retina of diabetic NOD1 chimeras. A Flow cytometry gating strategy for Ly6C^lo and Ly6C^hi monocytes in bone marrow. B Flow cytometry diagrams of bone marrow monocytes from WT → WT, NOD1^−/− → WT, Akita → Akita and NOD1^−/−-Akita → Akita mice. C, D The percentages of Ly6C^lo and Ly6C^hi monocytes among CD45⁺ cells (flow cytometry analysis; n = 10–13 per group). E Flow cytometry gating strategy for bone marrow-derived monocytes (CD45^hiCD11b⁺Ly6G⁻Ly6C^hi), macrophages (CD45^hiCD11b⁺Ly6G⁻Ly6C^loF4/80^hi) and neutrophils (CD45^hiCD11b⁺Ly6G⁺) in pooled retina samples from the respective groups. F Representative images of neutrophils in the retina. G Ly6C^hi monocytes and Ly6C^loF4/80^hi macrophages in the retina. H–J Bar graphs showing the percentages of retinal Ly6C^hi monocytes, Ly6C^loF4/80^hi macrophages and neutrophils among CD45⁺ cells (n = 10 per group). Data are presented as the mean ± SD; *p < 0.05, **p < 0.01. WT, wild type; BM, bone marrow; MΦ, macrophage