Fig. 5

Rescue of oocyte senescence in aged mice by LRP1^high subpopulation. A PCA analysis demonstrates the transcriptome sequencing results of oocytes from young mice, aged mice, and aged mice with intraovarian transplantation of LRP1^high or LRP1^low subpopulation. B The heatmap displays the differentially expressed genes in oocytes from each group of mice. C The GO-BP and KEGG functional enrichment analysis of differentially expressed genes in oocytes from young and aged mice. D The oocyte aging scoring system was used to assess the degree of oocyte aging in each group of mice. The transplantation of LRP1^high subpopulation in aged mice effectively restored the level of oocyte aging, making it closer to that of young mice. Mean ± SD, *** P ≤ 0.001; ** P ≤ 0.01. E At the transcriptional level, the expression of aging genes in oocytes was evaluated after transplantation of different subpopulations of human UC-MSCs. Mean ± SD, n = 3, ** P ≤ 0.01; * P ≤ 0.05. The results showed that LRP1^high subpopulation could restore the expression of various pathway-related genes in oocytes of aged mice