Fig. 3
From: Short cell cycle duration is a phenotype of human epidermal stem cells
Cell cycle duration analyses of both live cell imaging and EdU incorporation reveal that expanding human keratinocyte colonies have shorter cell cycle durations than differentiating colonies. (A) Comparison of CCD of divisions in expanding vs. differentiating colonies. Mann-Whitney test; all divisions: 19 h (n = 1017) vs. 30 h (n = 174), P < 0.0001; PP: 19 h (n = 899) vs. 31 h (n = 63); PD: 22 h (n = 73) vs. 29.5 h (n = 54), P < 0.0001; DD: 26 h (n = 45) vs. 32 h (n = 57), P = 0.193. (B) Comparison of CCD of PP vs. PD vs. DD divisions. Dunn’s multiple comparison test (Kruskal-Wallis post-test). (C) CCD of all divisions in expanding vs. differentiating colonies over time. (D) CCD of PP divisions in expanding vs. differentiating colonies over time. (E) Median CCD for each division/generation (-TD5 to TD-1), for colonies that underwent different numbers of generations before terminal differentiation. (F) CCD of all divisions in the first 3 generations of expanding vs. differentiating colonies. Mann-Whitney test, P < 0.0001. (G) EdU in keratin10-Ki67+ cycling SCs and early CPs vs. keratin10+Ki67+ cycling CPs. Scale bar 50 μm. (H) Proportion of EdU+ (S-phase) cells in cycling CPs vs. SCs and early CPs in (G). t test, n = 5, P = 0.005. Data presented as median (95% CI) in A-F or mean ± SEM in H. (): divisions analyzed. EXP: expanding colonies. DIF: differentiating colonies. TD: terminal differentiation. FO: final observation. Not significant (ns) P > 0.05. *P < 0.05. ***P < 0.001