Fig. 7

Immunofluorescence stainings of bioprinted hPSC-CEnCs on fibrin membrane. Cells were bioprinted in cylindrical structures of two printed layers with 0.30 mm line distance. Experiment conducted with the hESC line. A Representative fluorescence microscope images of ZO-1 (green) and CD166 (red) expression from day 1, 4 and 7 after printing (n = 2 for every timepoint). Relatively thick fibrin membrane produces background noise to the images. B Representative confocal microscope ortho images of ZO-1 (green), CD166 (red), Na⁺/K⁺-ATPase (red) and Hoechst (blue) expression at day 7 after bioprinting (n = 2). Scale bars A 200 µm (in inserted magnified images 100 µm), B 100 µm