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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Cryopreserved cGMP-compliant human pluripotent stem cell-derived hepatic progenitors rescue mice from acute liver failure through rapid paracrine effects on liver cells

Fig. 1

Expression profile of differentiation markers in GStemHep cells. A Schematic diagram of PSC-derived GStemHep cells. Human PSC were differentiated into GStemHep cells with a 10-day protocol. The expression profile of the indicated markers was analyzed in cells and culture supernatants. Pluripotency, endoderm and hepatic markers are shown in blue, green and orange, respectively (except for immunofluorescence staining). B RT‒qPCR analysis of the expression of key markers for differentiation stages and commitment to liver fate in GStemHep (n = 10). The results were normalized to the GAPDH housekeeping gene and expressed as the fold change relative to PSC. C Representative FACS dot plot of a PSC and a GStemHep production batch (detection threshold of OCT4 flow cytometry is 0.2%). Cells incubated with isotype control are in gray. D Average expression of pluripotency factor (OCT4) and hepatoblast markers (SOX17/HNF4A/AFP) by GStemHep (n = 9). E Immunofluorescence staining of GStemHep and human PSC for pluripotency (OCT4), proliferation (Ki67), endoderm (SOX17) and hepatic markers (HNF4A et AFP). The marker proteins and DAPI are shown in green and blue, respectively (magnification 20x). F Detection of secreted human AFP and human HGF in culture supernatant by ELISA (n = 10) (***p = 0.0007 Mann‒Whitney test)

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Stem Cell Research & Therapy

ISSN: 1757-6512