Fig. 3
Therapeutic effect of GStemHep in NOD/SCID mice with APAP-induced acute liver failure. After APAP intoxication, mice were treated (APAP + GStemHep) or not (APAP only) with intrasplenic injection of 1 × 106 thawed GStemHep. Mouse survival was followed over 10 days, or mice were euthanized at 3 h, 6 h and 24 h post treatment for analysis of liver damage markers. A Survival curve of APAP-induced ALF mice treated with GStemHep injection (n = 46) compared with non-treated group (n = 60) (****p < 0.0001 log-rank (Mantel‒Cox) test). B Biochemical analysis of liver damage markers: ASAT (up) and ALAT (down) in the serum of each group (n = 18 for APAP only, n = 19 for APAP + GStemHep and n = 8 for no APAP) 24 h after APAP intoxication (*p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001 one-way ANOVA test). C Necrosis quantification on HES-stained liver sections in APAP only (n = 5) and APAP + GStemHep groups (n = 5) at 3 h, 6 h and 24 h after GStemHep transplantation (**p < 0.01 two-way ANOVA test). D Representative HES-stained liver sections from each group (n = 5 per group) 24 h after GStemHep transplantation (magnification 5x). Areas of necrosis are delimited by the red dotted lines