Fig. 4

GStemHep tracking in the APAP-ALF model after transplantation. After APAP intoxication and cell transplantation (APAP + GStemHep), cell homing was analyzed in the liver and the spleen, and human AFP were quantified in the serum of mice at different times post-treatment (3 h, 6 h and 24 h). Untreated APAP-ALF mice served as controls (APAP only). A Detection of human ALU DNA sequences (human specific/290 bp) in mouse liver by PCR at 24 h after transplantation; each number represents a different mouse. B Immuno-histochemical staining for specific human Ku80 in mouse liver and spleen at 24 h after transplantation (Magnification 10 × on the left and 20 × on the right), data representative of 10 analyzed mice. (C) Quantification of human AFP in mouse serum by ELISA 3 h, 6 h and 24 h after transplantation (n = 4 in APAP only, n = 5 in APAP + GStemHep at 6 h and 9 h, n = 14 in APAP + GStemHep at 24 h post-transplantation), each point represents a different mouse