Fig. 7
Therapeutic effect of GStemHep and cell tracking in C57Bl/6 mice with TAA-induced ALF. After TAA intoxication, mice were treated (TAA + GStemHep) or not (TAA only) with intrasplenic injection of 1 × 106 GStemHep. Mouse survival was monitored for 7 days, or mice were euthanized at 24 h post treatment for PCR analysis of the liver. Sera were collected at different times. A Survival curve of TAA-induced ALF mice treated with GStemHep (n = 101) compared with non-treated group (n = 80) (****p < 0.0001 log-rank (Mantel‒Cox) test). B Quantification of human AFP in the serum of the TAA-only group (n = 5) and the TAA + GStemHep group at 24 h (n = 13) and 7 days (n = 5) after GStemHep transplantation by ELISA. C Detection of human ALU DNA sequences in mouse livers by PCR at 24 h after transplantation; each number represents a different mouse