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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production

Fig. 3

Trilineage differentiation potential of HFP-MSCs. (A) Adipogenic differentiation of HFP-MSCs indicated by lipid droplets stained with Oil Red O from a representative donor. Scale bar 100 μm. (B) Colorimetric quantification of Oil Red O extracted from lipid droplets. (C) Osteogenic differentiation indicated by deposition of calcified material stained with Alizarin S. (D) Colorimetric quantification of Oil Red O extracted from calcified material. (E) Chondropellet formation in presence of differentiation medium supplemented with FCS, CPRP or hypACT. Scale bar 1 mm. Images were uniformly contrast enhanced using automatic white correction via GIMP 2.8.22 software (F) Diameter of chondropellets in presence of FCS, CPRP or hypACT. Data are represented as mean ± SD. n = 3–5, * . p < 0.05, ** . p < 0.005, *** . p < 0.001, **** . p < 0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512