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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production

Fig. 6

Characterisation of particle size and concentration as well as particle recovery efficiency and correlation with protein amount via NTA after enrichment via ultrafiltration. (A) Analysis of particle and protein concentration in fractions after ultrafiltration (UF) from a representative batch of 3 bioreactors operated in parallel under different treatment conditions (FCS, CPRP, hypACT). (B) Particle size of EV preparations from 4 donors in each of the indicated treatment conditions. Data are given as mean ± SD. (C) Recovery efficiency of particle enrichment via UF comparing amount of particles in input conditioned supernatant and amount of particles in final EV preparation. Data are given as mean ± SD. * . p < 0.05. (D-F) Correlation analysis of particle and protein concentrations in UF fractions (EV, W1-W5) from 4 donors

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Stem Cell Research & Therapy

ISSN: 1757-6512