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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production

Fig. 7

Characterisation of EV marker protein presence via Western Blot and EV marker protein colocalisation via fluorescence NTA. (A) Detection of EV markers CD9, CD63 and Alix as well as the negative marker ApoB100 indicating presence of EVs and absence of contaminants from blood product treatments via Western Blot. (B) Representative result of colocalisation of CD9+ (green) and CD73+ (red) particles via fluorescence NTA. Blue crosses indicate colocalisation. (C) Left, quantitative assessment of CD9+ EVs colocalising with CD73 and CD73+ EVs colocalising with CD9 as determined via NTA in fluorescence mode. Right, comparison of mode size of CD9+ and CD73+ single positive EVs. Data are given as mean ± SD of a representative donor. **** . p < 0.0001, ** . p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512