Fig. 5

Gene Set Enrichment Analysis (GSEA) comparison of the biological processes’ pathways of fMSCs and aMSCs. (A, B) Bar chart of the top 15 significantly enriched pathways in fMSCs (A) and aMSCs (B). (C, D) Normalized enrichment plots of novel pathways/gene sets enriched in fMSCs (C) and aMSCs (D). The top part of each plot shows the enrichment score that represents running-sum statistic calculated by “walking down” the ranked list of genes. The green line represents the time-course gene expression data (the normalized enrichment score) and the vertical black lines indicate the position of the genes found in the target gene set within a gene list ranked by log2 fold changes. The Enrichment score is the maximum deviation from zero as calculated for each gene going down the ranked list and represents the degree of over-representation of a gene set at the top or the bottom of the ranked gene list. The coloured bar at the bottom of the plot shows positive (red) and negative (blue) correlation to phenotype in fMSCs and aMSCs (C, D)