Fig. 1
Hematopoietic differentiation for HSC generation from human induced pluripotent stem cells. A schematic protocol of hiPSC differentiation into HSPCs with chemically defined factors. Cells were treated with either a diarylheptanoid ASPP 049 or vehicle control (DMSO) starting on day 7 with medium changes. B Representative morphologies of differentiating cells in adherent (black arrow) and nonadherent (black arrowhead) fractions during the differentiation: scale bars, 100 μm