Fig. 2

EV characterization. A Single EV visualized as a donut-shaped particle on TEM. B/C. Size distribution of hiPSC-EVs (B) and diPSC-EVs (C) as measured by Nanoparticle Tracking Analysis (NTA). D/E. Imaging flow cytometry (negative controls and hiPSC-EVs). Results of co-staining with antiCD81-FITC (Ch02) and antiCD63 (Ch03) are presented in D. EV gating with CFDA-SE (Ch02) followed by co-staining with antiCD63 (Ch03) is shown in E