Fig. 2

iMSC^ACVR2B-Fc-conditioned medium inhibited BMP and TGF-β signaling in FOP-iMSCs. A, B iMSC^ACVR2B-Fc-conditioned medium inhibited the BRE-Luc activity in FOP-iMSCs (induced by Activin-A and BMP-9) and in resFOP-iMSCs (induced by BMP-9). C, D iMSC^ACVR2B-Fc-conditioned medium downregulated the expression of ID1 (a downstream gene of the BMP pathway) in FOP-iMSCs (induced by Activin-A and BMP-9) and in resFOP-iMSCs (induced by BMP-9). E Representative image of western blotting analysis. iMSC^ACVR2B-Fc-conditioned medium inhibited SMAD1/5/8 phosphorylation (induced by Activin-A and BMP-9) in FOP-iMSCs. All full-length blots are presented in Additional file 2: Fig. 2E. F Quantification of relative p-SMAD1/5/8 levels, normalized to β-actin. G iMSC^ACVR2B-Fc-conditioned medium inhibited the CAGA-Luc activity (induced by Activin-A) in resFOP-iMSCs and FOP-iMSCs. H iMSC^ACVR2B-Fc-conditioned medium downregulated the expression of CTGF (a downstream gene of the TGF-β pathway; induced by Activin-A) in FOP-iMSCs. I Representative image of western blotting analysis. iMSC^ACVR2B-Fc-conditioned medium inhibited SMAD2/3 phosphorylation (induced by Activin-A) in FOP-iMSCs. All full-length blots are presented in Additional file 2: Fig. 2I. F Quantification of relative p-SMAD2/3 levels, normalized to β-actin. Results represent the mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-way ANOVA with Šídák's multiple comparisons test. Activin-A, 100 ng/ml; BMP-9, 10 ng/ml