Fig. 10

MEA recordings of Anatomic RealDRG™ sensory neurons over the maturation period. A Cells were plated over the electrode array region within a typical well of the Axion 48 well cytoview MEA plate shown here at DIV16 (16 electrodes/well). Scale bar—100 µm. B Data visualization from RealDRGs cultured on MEAs. Heat plot across the 48 well MEA indicating spike activity. Each colored circle is an electrode with detected APs. C Representative traces showing a single active electrode before and during a 42 °C temperature ramp at DIV36. D Representative raster plots tracking the per-electrode responsiveness to 42 °C heat ramp from a single timepoint each during week 2, 3, 4, and 5. E The percent active electrode yield (AEY) from 384 electrodes in a 48-well plate for three recording sessions each during week 2, 3, 4, and 5 after plating of cells. Blue trace describes the AEY during 42 °C trials, the 37 °C electrode data (prior to heating) is shown in black. F The average Mean Firing Rate (MFR, Hz, mean ± SEM) for 384 electrodes in a 48-well plate for three recording sessions each during week 2, 3, 4, and 5 after plating of cells. Blue bars describe the MFR during 42 °C trials at each timepoint, the 37 °C trials MFR is shown in black. Asterisks denote significance with Sidak’s multiple comparisons (**p < 0.01, ***p < 0.001, ****p < 0.0001). G Pie charts describing the evolution of responsiveness to 42 °C from the 174 active electrodes identified at week 5 from their response in the previous weeks. The four types of responses during week 5 were: consistent (positive response from 3 of 3 42 °C trials/wk), responders (1 ≤ positive trials < 3 trials/wk), negative (spontaneously active with negative responses to 42 °C), and inactive electrodes (< 1 spk/min)