Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway

Fig. 4

MSC-Mito coadministration of PQQ ameliorates chemotherapy-induced POI through the SIRT1/ATM/p53 pathway. (A-B) Comet assays were employed to assess the impact of SIRT1 knockdown on the DNA damage induced by PQQ and MSC-Mito. (C-D) Flow cytometry was utilized to evaluate the influence of SIRT1 knockdown on apoptosis induced by PQQ and MSC-Mito. (E) The effects of SIRT1 knockdown on oxidative stress induced by PQQ and MSC-Mito were examined. (F) Western blot analysis was conducted to investigate the influence of SIRT1 knockdown on mitochondrial biogenesis induced by PQQ and MSC-Mito. (G) Western blotting was employed to assess the impact of SIRT1 knockdown on the ATM/p53 signalling pathway induced by PQQ and MSC-Mito. (H) Quantitative analysis of Western blot results. Scale bar: 10 μm. Values are the means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512