Fig. 4

ELK inhibits lymphocyte differentiation and promotes myeloid differentiation. A A schematic showing the experimental design used for the analysis of ALA on HSPC lineage differentiation in vivo. B Representative flow cytometry plots of pCCL-L-ELK1 (L-ELK1), pCCL-S-ELK1 (S-ELK1), and pCCL-GFP (Ctrl) lentiviral-transduced CD34⁺ HSPCs 48 h after transduction. C–D Representative flow cytometry plots of hCD19⁺ B cells, hCD11b⁺ myeloid cells, hCD15⁺ neutrophils, and hCD14⁺ monocytes gated from single hCD45⁺GFP⁺ live cells derived from the bone marrow of M-NSG mice. M-NSG mice were sacrificed 3 months after transplantation. E Representative flow cytometry plots of hCD123^high dendritic cells (DC) and CD123^mid CD34^low/+ CD15⁺ CD64^mid neutrophil progenitors gated from single GFP⁺ live cells derived from the bone marrow of M-NSG mice. M-NSG mice were sacrificed 3 months after transplantation. F–G Statistical analysis of hCD19⁺ B cells, hCD11b⁺ myeloid cells, hCD15⁺ neutrophils, and hCD14⁺ monocytes gated from hCD45⁺GFP⁺ single live cells derived from the bone marrow of M-NSG mice. M-NSG mice were sacrificed 3 months after transplantation. H Statistical analysis of hCD123^high DC and CD123^mid CD34^low/+ CD15⁺ CD64^mid neutrophil progenitors gated from single hCD45⁺ GFP⁺ live cells derived from the bone marrow of M-NSG mice. M-NSG mice were sacrificed 3 months after transplantation. I Flow cytometry analysis of Lin^–c-Kit⁺Sca1^–CD16/32⁺CD34⁺ GMP, Lin^–c-Kit⁺ Sca1^– CD16/32^mid CD34^low CMP, Lin^–c-Kit⁺Sca1^–CD16/32^– CD34^– MEP, CD71⁺Ter119⁺ erythrocyte progenitors, CD71^–Ter119⁺ erythrocytes, CD11b⁺ myeloid cells, CD11b⁺ Ly6G⁺ Ly6C^mid mature neutrophils, CD11b⁺ Ly6G^–/low Ly6C^mid immature neutrophils and CD11b⁺ Ly6G^– Ly6C^high monocytes in bone marrow of Mx1-Elk1and littermate Ctrl mice. Presentative cytometry plots and statical plots are shown. The flow cytometry assays were performed on day7 post the treatment of PolyIC. The data in panels (F–H) are presented as the mean ± SD. An unpaired Student’s t-test (two-tailed) was performed. N = 3–4 individuals; n.s, no significance; **P < 0.01, ***P < 0.001, ****P < 0.0001