Fig. 2

(A) Cell cycle analysis of MDA-MB-231 cells treated for 48 h with control (DMEM-F12 without FBS), CM-hUCESC, paclitaxel (1 µM) or the combination of paclitaxel (1 µM) + CM-hUCESC, and then subjected to flow cytometry using propidium iodide (PI). Percentage of cells (mean + standard deviation) in each phase is shown. (B) Apoptosis was determined in MDA-MB-231 cells cultured for 48 h with control (DMEM-F12 without FBS), CM-hUCESC, paclitaxel (1 µM) or the combination of paclitaxel (1 µM) + CM-hUCESC, by flow cytometry using Annexin V/PI. Annexin V+/PI- and Annexin V+/PI + indicated early and late apoptosis, respectively. (C) Invasive capacity of MDA-MB-231 cells treated for 48 h with control (DMEM-F12 without FBS), CM-hUCESC, paclitaxel (1 µM) or the combination of paclitaxel (1 µM) + CM-hUCESC in Matrigel invasion chambers. (D) Invasive capacity of MDA-MB-231 cells treated for 48 h with control (DMEM-F12 without FBS), CM-hUCESC, IgG IP and CM-hUCESC lacking TIMP-1 and − 2 (IP TIMP-1/2) in Matrigel invasion chambers. Data represent the mean ± SD. *p < 0.05; ***p < 0.0001