Fig. 3
From: Cell-specific extracellular vesicle-encapsulated exogenous GABA controls seizures in epilepsy
Characterization of isolated extracellular vesicles. Figure 3A represents the size distribution of isolated EVs demonstrated by Nanosight LM10 for neural stem cell-derived EVs (NSC-EVs), medial ganglionic eminence cell-derived EVs (MGE-EVs) and interneuron derived EVs (IN-EVs). Figure 3B represents TEM images of isolated NSC-EVs, MGE-EVs and IN-EVs with a scale bar of 200 nm. Figure 3C demonstrates that NSC-EVs, MGE-EVs and IN-EVs are positive for EV cytoplasmic marker TSG 101 and EV transmembrane marker CD 63. At the same time, they are mostly negative for GRP 94, an endoplasmic reticulum marker. The presence of β-actin is demonstrated for all the markers. Cells are positive references for CD63, TSG101 and GRP94 in all the groups