Fig. 2
From: Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system
Investigation of the expression timing of FEV during SN differentiation. A Schematic diagram of the strategy to derive SN from the FEV reporter hPSC. B Immunofluorescence staining of SOX1, GATA2 and NKX2.2 to indicate reporter cell line (H9-Clone#7)-derived SNP at day 21 of differentiation. C. Immunofluorescence staining of EGFP and TPH2 to validate the expression time of FEV and TPH2. D, E Time course of FEV (D) and TPH2 (E) expression during SN differentiation process. F Immunofluorescence staining of GATA3 at day 42 of differentiation. G Immunofluorescence staining of EGFP and 5-HT at day 56 of differentiation. Scale bar for B, C, F, G 100 μm. n = 3 independent experiments. All data are presented as means ± SEM