Fig. 6

Exploration of the key signaling pathways during SN differentiation. A Heatmap plot of topological overlap matrix (TOM) in the gene network. Rows and columns correspond to single genes, light color represents low overlap and progressively darker red color represents higher overlap. Blocks of darker colors along the diagonal are the modules. B The correlation of DEGs in modules across key stages of SN differentiation. C PPI analysis of the overlapping DEGs exclusively expressed on day 28 and in module brown. D PPI analysis of the overlapping DEGs exclusively expressed on day 42 and in module pink. E Expression patterns of the serotonergic-associated genes across individual serotonin neurons presented as UMAP plots. F Schematic of the signaling pathways and molecules that paly essential roles during SN differentiation