Figure 3

Flow cytometry analysis of cell surface markers on passaged 2 (P2) BMSCs after culture in α-MEM comprising 10% FBS and 5 ng/ml FGF-2. (A) Representative fluorescence intensity histograms are presented showing expression of cell surface markers of normoxia- and hypoxia-expanded BMSCs at P2 of a 43-year-old man (BM74). (B-D) The mean fluorescence intensity (MFI) of cell surface markers on BMSCs from: (B) a 34-year old woman (BM68), (C) a 43-year-old man (BM74), (D) a 62-year-old man (BM84). (E) Represents the mean fluorescence intensity ± standard error of three donors; BM68, BM74 and BM84. *P < 0.05 in normoxia versus hypoxia, Student's t test. BMSCs, bone marrow mesenchymal stromal cells; FBS, fetal bovine serum; FGF-2, fibroblast growth factor-2; MEM, modified Eagle's medium.