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Table 2 Conventional cytogenetic analysis on hBM-MSCs from eight healthy donors at several passages in vitro

From: From cytogenomic to epigenomic profiles: monitoring the biologic behavior of in vitro cultured human bone marrow mesenchymal stem cells

Donor Passage Norm. karyotype
(%)
Clonal aneupl.
(%)
Clonal struct.
abnorm (%)
Random loss (%) Random gain (%) Metaphases (number) Metaphases/Cells (number) Mitotic index (%)
1
(46,XX)
7 45.5 0 0 54.5 0 22 24/1038 2.3
  9 66.7 0 0 33 0 39 8/1000 0.8
  12a 60 0 0 40 0 5 15/1000 1.5
2
(46,XY)
6 82.8 0 0 15.6 1.6 64 70/1000 7.0
  10 80 0 0 20 0 30 24/1010 2.4
  16a 45.5 0 0 36.4 18.1 11 11/1007 1.1
3
(46,XX)
3 70 0 0 26 4 23 4/1032 0.4
4
(46,XX)
3 78 0 0 22 0 27 16/1000 1.6
  6 70.4 0 0 25.9 3.7 27 16/1000 1.6
  9 41.2 52.9 (47,XX,+7) 0 5.9 0 17 2/1320 0.2
  12 30.6 50 (47,XX,+7) and 11.1 (46,X,+7) 0 8.3 0 36 16/1000 1.6
5
(46,XX)
4 33.3 33.3 (49,XX,+5,+7,+9) 0 33.3 0 18 12/1011 1.2
  6 80 0 0 12 8 25 7/1000 0.7
  10 81.3 0 0 18.7 0 32 14/1080 1.3
  12 92.3 0 0 7.7 0 26 18/1028 1.8
6
(46,XY)
4 86 0 0 14 0 50 47/1000 4.7
  6 76.5 0 0 23.5 0 17 2/1001 0.2
  9 82.7 0 0 17.3 0 53 41/1000 4.1
  12a 72.7 0 0 18.2 9.1 11 1/1000 0.1
7
(46,XX)
4 90.6 0 0 3.1 6.3 32 49/1011 4.8
  6 92.9 0 0 7.1 0 14 2/1000 0.2
  9 81.3 0 0 18.7 0 16 11/1029 1.1
8
(46,XY)
4 100 0 0 0 0 37 27/1030 2.6
  6 78.1 0 0 17 4.9 41 22/1088 2.0
  9 77 0 0 23 0 30 6/1000 0.6
  1. aIn senescence. Clonal aneupl., clonal aneuploidies; Struct. abnorm., structural abnormalities.