Figure 2

Extracellular matrix and adhesion molecules gene expression in LSK from different microenvironments of development. (a-f) Volcano plots comparing the extracellular matrix and adhesion molecules gene expression fold change between pairs of samples (reference sample indicated in the title of each plot). Each dot represents the expression of one gene. Dots above the horizontal line show significant difference (P < 0.05). Dots to the right of the vertical line show fold increase while dots on the left show fold decrease (above a value of two). FI, fold increase, FD, fold decrease. Central vertical line represents a fold value of zero. Red squares highlight significant fold decrease, while blue squares indicate significant fold increase. (g) Expression fold change of genes that showed significant difference between FL14.5, FL17.5, FBM17.5 and adult BM. Genes analyzed include Col4a1, Entpd1, Mmp2, Sell, Tgfbi, and Timp2. Gene expression was normalized to adult BM. Note: * = significant difference (P < 0.05) with BM; † = significant difference (P < 0.05) with FBM17.5; ‡ = significant difference (P < 0.05) with FL17.5.