Skip to main content
Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Monitoring mitochondrial inner membrane potential for detecting early changes in viability of bacterium-infected human bone marrow-derived mesenchymal stem cells

Figure 1

Total dissipation of human bone marrow-derived mesenchymal stem cells (BMMSCs) by bacterial infection. BMMSCs (line 412) were infected with bacteria, and after 24-hour incubation the cells were stained with Trypan blue and studied microscopically. (a) Control cells were viable after 24 hours of cultivation. (b) Pseudomonas aeruginosa PA01-infected BMMSCs show total dissipation at 24 hours with all bacteria loads. (c) Staphylococcus aureus Cowan I and (d) S. aureus KK1089 formed enormous aggregates and caused the decrease in viability as determined by Trypan blue. (e) Staphylococcus epidermidis ATCC 14990 and (f) S. epidermidis KK1087 showed total dissipation of BMMSCs at 24 hours of infection. Magnification was 200 × in all panels. Red circles indicate Trypan blue-positive nuclei. Red asterisks indicate the aggregates produced by S. aureus.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512