Implanted Mst KO MDSCs stimulate more effectively than do WT MDSCs the expression of MHC-II in the muscle, but do not reduce ASMA. (A) Western blot analysis for MHC II, ASMA, and GAPDH (reference gene) in homogenates of skeletal muscle tissue from the central region adjacent to area examined histochemically in Figure 7C. Each lane corresponds to an individual mouse homogenate (n = 5/group), and the three gels were run simultaneously. (B) Densitometric evaluation of the relative intensity expressed as ratios of the MCH-II or ASMA and GAPDH bands. *P < 0.05. Mst KO, myostatin knockout; MDSC, muscle-derived stem cell; WT, wild type; MHC, myosin heavy chain; ASMA, α-smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.