Figure 5

BV2 and MSCs are migratory toward each other. Migration was assessed by counting the number of cells stained with crystal violet on the converse side of transwell culture inserts. (A) Migration for BV2 microglia was assessed at 6 hours. Values are expressed as mean cell number ± SD and from three independent experiments. **P < 0.01, ***P < 0.001 compared with control. (B) Supernatants from 24-hour unprimed and primed MSCs were transferred to BV2 cultures to assess BV2 migration at 6 hours. Values are expressed as mean cell number ± SD and from four independent experiments. (C) Migration for MSCs was assessed at 24 hours. Values are expressed as mean cell number ± SD and from three independent experiments. *P < 0.05, ***P < 0.001 compared with control. (D) CCL2 levels were assayed at 24 and 48 hours in culture supernatant with the BD Cytometric Bead Array. Values are expressed as mean and from a representative of three independent experiments. CCL2, chemokine (C-C motif) ligand 2; MSC, mesenchymal stem cell; SD, standard deviation.