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Table 2 Culture media formulations used for differentiation assays

From: Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells

Medium Basal media Serum Coating/coating-free supplements Supplementation
Adipogenic (FBS) DMEM/F-12 10% Fetal bovine serum None 1% GlutaMAX, 1% p/s, 33 μM biotin (Sigma), 1 μM dexamethasone (Sigma), 100 nM insulin (Life Technologies), 17 μM pantothenate (Fluka, Buchs, Switzerland), 250 μM isobutylmethylxanthine (IBMX; Sigma) for 48-hour induction after cell seeding
Osteogenic (FBS) DMEM/F-12 10% Fetal bovine serum None 1% GlutaMAX, 1% p/s, 150 μM L-ascorbic acid 2-phosphate (Sigma), 10 mM β-glycerophosphate (Sigma), 10 nM dexamethasone (Sigma)
Chondrogenic (FBS/HS) DMEM/F-12 None None 1% GlutaMAX, 0.3% p/s, 10 mg/ml human serum albumin (Sigma), 8 μg/ml holo-transferrin human (Sigma), 5 ng/ml sodium selenite (Sigma), 10 μg/ml insulin (Life Technologies), 1 μg/ml linoleic acid (Sigma), 50 μM L-ascorbic acid 2-phosphate (Sigma), 55 μM sodium pyruvate (Life Technologies), 23 μM L-proline (Sigma), 10 ng/ml TGF-β (Sigma)
Adipogenic (HS) DMEM/F-12 10% Human serum None 1% GlutaMAX, 1% p/s, 33 μM biotin, 1 μM dexamethasone, 100 nM insulin, 17 μM pantothenate, 250 μM IBMX for 48-hour induction after cell seeding
Osteogenic (HS) DMEM/F-12 10% Human serum None 1% GlutaMAX, 1% p/s, 150 μM L-ascorbic acid 2-phosphate, 10 mM β-glycerophosphate, 10 nM dexamethasone
Adipogenic (XF/SF CS) StemPro MSC none CELLstart™ coating StemPro MSC SFM XenoFree supplement, 1% GlutaMAX, 0.3% p/s, 33 μM biotin, 1 μM dexamethasone, 100 nM insulin, 17 μM pantothenate, 250 μM IBMX for 48-hour induction after cell seeding
SFM
Osteogenic (XF/SF CS) StemPro MSC none CELLstart™ coating StemPro MSC SFM XenoFree supplement, 1% GlutaMAX, 0.3% p/s, 150 μM L-ascorbic acid 2-phosphate, 10 mM β-glycerophosphate, 10 nM dexamethasone
SFM
Chondrogenic (XF/SF CS and XF/SF CM) StemPro MSC None None 1% GlutaMAX, 0.3% p/s, 10 mg/ml human serum albumin, 8 μg/ml holo-transferrin human, 5 ng/ml sodium selenite, 10 μg/ml insulin, 1 μg/ml linoleic acid, 50 μM L-ascorbic acid 2-phosphate, 55 μM sodium pyruvate, 23 μM L-proline, 10 ng/ml TGF-β
SFM
Adipogenic (XF/SF CM) StemPro MSC None Coating Matrix Kit StemPro MSC SFM XenoFree supplement, 1% GlutaMAX, 0.3% p/s, 33 μM biotin, 1 μM dexamethasone, 100 nM insulin, 17 μM pantothenate, 250 μM IBMX for 48-hour induction after cell seeding
SFM
Osteogenic (XF/SF CM) StemPro MSC None Coating Matrix Kit StemPro MSC SFM XenoFree supplement, 1% GlutaMAX, 0.3% p/s, 150 μM L-ascorbic acid 2-phosphate, 10 mM beta-glycerophosphate, 10 nM dexamethasone
SFM