Figure 3
Protein expressions of pro-angiogenic factors in ischemic skeletal muscle on Day 14 following CLI induction. A) In DPP4-deficient animals, notably higher eNOS expression in normal control (DPP4D-NC) than in DPP4D-CLI and DPP4D-CLI-GCF. * vs. †, P < 0.05. In wild-type (WT) Fischer rats, notably higher in normal controls (WT-NC) and WT-CLI-GCSF rats than in WT-CLI animals without difference between WT-NC and the WT-CLI-GCSF group. †vs. ‡, P < 0.01. B) In DPP4-deficient animals, notably higher VEGF expression in those after CLI induction with GCSF treatment than in those without and the non-CLI group (DPP4D-NC), and higher in DPP4-deficient rats after CLI induction than in DPP4D-NC. * vs. †vs. ‡, P < 0.01. In WT Fischer rats, significantly higher in WT-CLI-GCSF group than in WT-CLI and WT-NC, higher in WT-CLI than in WT-NC. * vs. †vs. ‡, P < 0.005. C) In DPP4-deficient animals, significantly higher CXCR4 expression level in DPP4D-CLI-GCSF and DPP4D-NC than in DPP4D-CLI, without difference between the former two groups. * vs. †, P < 0.03. In WT Fischer rats, remarkably higher in WT-CLI-GCSF than in WT-CLI and WT-NC, and higher in WT-CLI than in WT-NC. * vs. †vs. ‡, P < 0.01. D) In DPP4-deficient animals, significantly lower stromal cell-derived factor (SDF)-1α protein expression in DPP4D-CLI than in DPP4D-NC and DPP4D-CLI-GCSF group, without difference between the latter two groups. * vs. †, P < 0.04. In WT Fischer rats, markedly increased in WT-CLI-GCSF than in WT-CLI and WT-NC, and higher in WT-CLI than in WT-NC. * vs. †vs. ‡, P < 0.001. A-D) Statistical analysis by one-way ANOVA. * vs. other groups, P < 0.001. Symbols (*, †, ‡, §) indicate significant difference (at 0.05 level) by Tukey's multiple comparison procedure (n = 6 per group).