Figure 5

Immunostained images of each group at 21 days after transplantation. Phase-contrast microscopic images of spinal cord from each group (a, e), and transplanted cells labeled with PKH-26 (b, f, g) are shown. In group CL, clustering of the migrated PKH-26-positive cells was observed around the SCI lesion (f, g). Immunostained images of each group are shown © NF-H in group 1G, (d) GFAP in group 1G, (h) NF-H in group CL, (i) GFAP in group CL). Green staining indicates cells positive for the differentiation marker. Yellow-staining cells indicate grafted cells expressing each neural marker. Transplanted cells had a tendency to express higher levels of GFAP than NF-H in group 1G and in group CL (c, d, h, i). Scale bars, 300 μm (b and f), and 100 μm (c, d, g, h, and i).