Figure 3

Oxidative stress regulates cardiac gene expression in mesenchymal stem cells (MSCs). MSCs were treated with either H₂O₂ for 1 week (0 to 100 μM; A, B) or GOX for 48 hours (0 to 5 mU/ml; C, D). Expression of the cardiac markers αMHC (A, C) and nkx2-5 (B, D) was determined with qRT-PCR. Values are expressed as mean ± SEM after normalizing gene expression to GAPDH (A, B) or 18S (C, D) expression. For A and B, n ≥ 4; C and D, n ≥ 5. *P < 0.05 and ***P < 0.001 when compared with control (0 μM H₂O₂ or 0 mU/ml GOX) with one-way ANOVA followed by Dunnett posttest.