Figure 3

Identification of diabetes-response genes in adult OB and HPC neurons. (A) qPCR analysis of diabetes-response genes related to Wnt signaling. WT (wild-type) HPC NSCs, DB (diabetic) HPC NCSs, WT OB NSCs, and DB OB NSCs were differentiated simultaneously into neuronal lineages, and the total RNAs were extracted 24 hours after the neuronal induction (neuronal progenitor stage; NP). (B) qPCR analysis of diabetes-response genes in OB and HPC neurons. (C) Western blotting analysis of diabetes-response genes. Nrxn1 (neurexin 1) exhibited DB-specific downregulation (top). Similarly, Scn1b (sodium channel, voltage-gated, type I, β subunit) shows diabetes-associated reduction (middle). Relative band intensity of each protein in the Western blotting analysis is shown on the right.