Upregulated EAAT3 interacts with insulin in diabetes in OB. (A) IHC analysis of EAAT3 and Insulin in OB. IHC images using antibodies of EAAT3 (red) and Insulin (green) are shown. (B) Magnified image at dotted line area in Figure 5A is shown in separate panels. (C) IP analysis of EAAT3 and insulin in OB. The association between EAAT3 and insulin was specifically observed in the OB from diabetic rats when EAAT3 expression was highly upregulated.