Figure 6

Preconditioned dmMSCs enhance angiogenesis in diabetic heart. (A through D) Blood-vessel density of cells expressing smooth muscle actin along with corresponding quantification in (E); scale bar about 20 μm. (F through J) Quantification of eNOS-positive cells in all the groups. Group II versus group IV, ^#P < 0.05; ^##P < 0.01; and ^###P < 0.001; group III versus group IV, ^φP < 0.05; ^φφP < 0.01; and ^φφφP < 0.001. (K) Gene-expression analysis of eNOS and iNOS in all groups with RT-PCR. (L) Quantification of gel bands by using Image J software. Group II versus group III, *P < 0.05; **P < 0.01; and ***P < 0.001; Group II versus group IV, ^#P < 0.05; ^##P < 0.01; and ^###P < 0.001; group III versus group IV, ^φP < 0.05; ^φφP < 0.01; and ^φφφP < 0.001.