Table 1 Primer sequences
Genes | Product size (bp) | Sequences (5′ to 3′) |
|---|---|---|
GAPDH (f) | 370 | CTCTTGCTCTCAGTATCCTTG |
GAPDH (r) | GCTCACTGGCATGGCCTTCC | |
IGF-1 (f) | 166 | AGGCTATGGCTCCAGCATTC |
IGF-1 (r) | AGTCTTGGGCATGTCAGTGTC | |
HGF (f) | 167 | TCACACAGAATCAGGCAAGACT |
HGF (r) | AAGGGGTGTCAGGGTCAA | |
SDF-1 (f) | 122 | CGGCTGAAGAACAACAACAG |
SDF-1 (r) | GGGGGTCTACTGGAAAGTCC | |
FGF-2 (f) | 154 | TGTCTATCAAGGGAGTGTGTGC |
FGF-2 (r) | CAACTGGAGTATTTCCGTGACC | |
ANG1 (f) | 143 | GACACCTTGAAGGAGGAGAAAG |
ANG1 (r) | GTGTCCATGAGCTCCAGTTGT | |
VEGF (f) | 200 | ACCCCGACGAGATAGAGTACAT |
VEGF (r) | CTTCTAATGCCCCTCCTTGT | |
GATA-4 (f) | 90 | CCTCTCCCAGGAACATCAAA |
GATA-4 (r) | ACCCATAGTCACCAAGGCTG | |
NKx 2.5 (f) | 147 | GGTCTCAATGCCTATGGCTAC |
NKx 2.5 (r) | GTTCACGAAGTTGCTGTTGG | |
MEF2c (f) | 178 | CACGCCTGTCACCTAACATCC |
MEF2c (r) | TGTTAGCTCTCAAACGCCACAC | |
PCNA (f) | 110 | TGACCCTCACCGATACAACA |
PCNA (r) | CTGTACAGCACAGCCACGTT | |
p53 (f) | 157 | AGCATCTTATCCGGGTGGAAG |
p53 (r) | CCCATGCAGGAGCTATTACACA | |
CD31 (f) | 120 | CCCAGTGACATTCACAGACA |
CD31 (r) | ACCTTGACCCTCAGGATCTC | |
CD34 (f) | 200 | GGGTAGCTCTCTGCCTGATG |
CD34 (r) | TCTCTGAGATGGCTGGTGTG | |
NF-κB (f) | 200 | GCACCTGTTCCAAAGAGCAC |
NF-κB (r) | GTGGAGTGAGACATGGACACAC | |
iNOS (f) | 116 | GCTACACTTCCAACGCAACA |
iNOS (r) | CATGGTGAACACGTTCTTGG | |
eNOS (f) | 123 | TGACCCTCACCGATACAACA |
eNOS (r) | Â | CTGTACAGCACAGCCACGTT |